Saturday, March 2, 2013

anti-CD20 and anti-CD3 by Hu and Wen at Yale


Combination treatment with anti-CD20 and oral anti-CD3 prevents and reverses autoimmune diabetes

Abstract: Type 1 diabetes (T1D) is a T cell-mediated autoimmune disease, although B cells also play an important role in T1D development. Both T cell- and B cell-directed immunotherapies have shown efficacy in the prevention and reversal of T1D. However, whether the combined strategy of targeting both T and B cells could further improve the therapeutic efficacy remains to be explored. Herein we show that the combined treatment with intravenous anti-human CD20 (hCD20) and oral anti-CD3 significantly delayed diabetes development in pre-diabetic hCD20 transgenic NOD mice. More importantly, the combined treatment reversed diabetes in more than 60% of mice newly diagnosed with diabetes. Further mechanistic studies demonstrated that the addition of oral anti-CD3 to the B cell depletion therapy synergistically enhanced the suppressive function of Treg. Interestingly, the oral anti-CD3 treatment induced a fraction of IL-10-producing CD4 T cells in the small intestine through IL-10 and IL-27-producing dendritic cells. Thus, our findings demonstrated that combining anti-CD20 and oral anti-CD3 is superior to anti-CD20 monotherapy for restoring normoglycemia in diabetic NOD mice, providing important preclinical evidence for the optimization of B cell-directed therapy for type 1 diabetes.

12 comments:

  1. mercy is my name; i had suffered Diabetes for several years ago. My sugar level was as high as anything you can think about. i looked for how to get rid of this diabetes for a long period of time, but could not found any solution. Though i have always believed on herbal medicine, but was never chanced to look for any because of my work. But not too long i saw some good news on the internet on how a herbal doctor cured so many persons of various diseases, and viruses. So i also decided to contact the doctor those people talked about he is by the name Doctor Aba. I emailed him, and i explained my problem to him, he said he has the cure for diabetes and i decided to give him a try because there is no harm in trying. So i started the process, and he prepared some herbs and sent it to me here in my country, so i began to use it, and behold it worked for me like a magic. I am happy to say today i am diabetes free, and my sugar level is back to normalcy. Please if you also have same, and or similar problem please rush to contact Doctor Aba before it becomes too late for you. His email is dr.abaherbalhome@gmail.com you can whats App his number on +2348107155060

    ReplyDelete
  2. mercy is my name; i had suffered Diabetes for several years ago. My sugar level was as high as anything you can think about. i looked for how to get rid of this diabetes for a long period of time, but could not found any solution. Though i have always believed on herbal medicine, but was never chanced to look for any because of my work. But not too long i saw some good news on the internet on how a herbal doctor cured so many persons of various diseases, and viruses. So i also decided to contact the doctor those people talked about he is by the name Doctor Aba. I emailed him, and i explained my problem to him, he said he has the cure for diabetes and i decided to give him a try because there is no harm in trying. So i started the process, and he prepared some herbs and sent it to me here in my country, so i began to use it, and behold it worked for me like a magic. I am happy to say today i am diabetes free, and my sugar level is back to normalcy. Please if you also have same, and or similar problem please rush to contact Doctor Aba before it becomes too late for you. His email is dr.abaherbalhome@gmail.com you can whats App his number on +2348107155060

    ReplyDelete

  3. Am really happy that i was cured of Diabetes with the herbal medicine of Doctor Chukwu , i have been suffering from this disease for the past 6 years without solution until i came across his website http://drchukwuhealinghome.simplesite.com/ of this doctors who have cure so many people with his herbal medicine, i also choose to give him a chance to help me, he told me what to do and i kindly did it, and he gave me his herbal medicine and directed me on how to use it, i also follows his instruction for use and he ask me to
    go for a checkup after 3 weeks and which i did, to my greatest surprise my result came out as negative, and my sugar level is now ok, am really happy that there is someone like this Doctor Chukwu who is ready to help anytime any day. You can contact this Great man that cured my Diabetes permanently through his email: (drchukwu22@gmail.com ) website: http://drchukwuhealinghome.simplesite.com/
    number +2348154800222. Bless you sir for all your good works.

    ReplyDelete
  4. I want to say thanks to Doctor James Omoghene for helping me cure my diabetes type 2. Brethren, i have suffered diabetes for a long period of time, i have tried so many remedy, but known seems to work. But i had contact with a herbal doctor who i saw so many people testifying on how they were all cured of their various disease and viruses by this doctor. So i explained my entire problem to him, and he promised to cure me. So i gave him all benefit of doubt, and behold he prepared the herbal mixture, and send it to me in my country. Today, i am proud to say i am diabetes free, and my sugar level has been restored to normal. So in case you are out there suffering from diabetes and other disease and or virus, i want to tell you to quickly contact Dr James Omoghene for your cure. His email is (drjamesspelltemple001@gmail.com) you can also call him on (call +2348107460527)

    ReplyDelete
  5. I'm Dorothy Saltzman, From Texas, USA. but curently Reside in Germany. It's no longer Rumors that the Acquired Immune Deficiency Syndrome ALS And Herpes Virus is increasing Day by Day. The fear is that many people living with the sickness are scared of saying it because of the stigma that comes along with it. I 'm bold enough among many others to state that there is now a Potent Cure to this sickness but many are unaware of it. I discovered that I was infected with ALS virus 3months Ago, After a Herbal Medical Check-Up. My Doctor EHI, told me and I was shocked, confused and felt like my World has crumbled. I was dying slowly due to the Announcement of my Medical Practitioner but he assured me that I could leave a normal life if I took my Medications (as there was no Medically known Cure to Herpes). I went from Churches to Churches but soon found that my Case needed urgent Attention as I was growing lean due to fear of dying anytime soon. In a bid to look for a lasting solution to my Predicament, I sought for solutions from the Voodoo World. I went online and searched for every Powerful Trad-medical Practitioner that I could Severe, cos I heard that the African Voodoo Priests had a cure to the Herpes Syndrome. It was after a little time searching the Web that I came across one Dr EHI (A Powerful African Voodoo Priest), Who offered to help me at a Monetary fee. I had to comply as this was my Final bus-stop to receiving a perfect healing. My last resolve was to take my life by myself, should this plan Fail. At last it worked out well. He gave me some steps to follow and I meticulously carried out all his Instructions. Last month, to be precise, I went back to the hospital to conduct another test and to my Amazement, the Results showed that " I am NEGATIVE". You can free yourself of this Herpes virus by consulting this great African Voodoo Priest via this e-mail:(drehicurehome123@gmail.com) or call and whats-app him on +2349030589283 He will help you and his Herbal Medication is Sure. He has the Cure on all this Disease/Virus as He told me and it listed. GENITAL WARTS/ PREGNANCY. EPILEPSY. GENPILENCIN, HIV/AIDS, MADNESS, WEAK ERECTION, WATERY SPERM, TUBERCULOUSES, PENIS ENLARGEMENT, PREGNANCY, DIABETICS, STROKE, EXPANDS OF PENIS BREAST H.P.V TYPE 1 TYPE 2 TYPE 3 AND TYPE 4. TYPE 5, HUMAN PAPAILOMA VIRUS, HERPES, SYPHILIS, BREAST CANCER, HEPATITIS A B and C, HIGH BLOOD PRESSURE, AND BODY BOILS, BODY ODDUR, AND BODY DISEASES , THANK TO YOU ALL FOR YOUR TIME OF READING MY TESTIMONY AND MOST ESPECIALLY YOU Dr EHI. I PRAY TO GOD TO NEVER LET ME AND MY FAMILY FORGET YOU IN OUR LIFE

    Why go the Expensive Treatment? when Dr. EHI is there to help you get rid of that
    Incurable Disease with his Herbs and Healing Oil. I contracted Genital Herpes 2 years ago, I saw this Awesome Testimonies you can view on google during my research for Herpes Cure from an article made by Alexander Millin and Lancelot Morgan, How they but got Cured from HIV/HERPES VIRUS Respectively. They also dropped the Contact Address of this Great Man: drehicurehome123@gmail.com Advising that if anyone need help to Cure any Incurable Disease or Viral Infection that he should Contact This Great Man. I contacted him, thank God I did. I'm still in shock that the Herbal Treatment he sent to me actually Works. It seems so simple, and in a lots of ways I guess its simple. But the important thing is that I have been 100% herpes free for full two years now and it's all thanks to you Dr. Ehi You'hv given me my life back, I'll forever remain Grateful,. you can email me for assistant on: drehicurehome123@gmail.com. Please Once you are cured let others know about it no need hiding it. Together We fight to become healthy.
    Dr. EHI Root/Herbs and Healing-Oil has been used to cured Home and Abroad
    REVELATION22.2
    GENESIS1:29

    ReplyDelete
  6. I'm Dorothy Saltzman, From Texas, USA. but curently Reside in Germany. It's no longer Rumors that the Acquired Immune Deficiency Syndrome ALS And Herpes Virus is increasing Day by Day. The fear is that many people living with the sickness are scared of saying it because of the stigma that comes along with it. I 'm bold enough among many others to state that there is now a Potent Cure to this sickness but many are unaware of it. I discovered that I was infected with ALS virus 3months Ago, After a Herbal Medical Check-Up. My Doctor EHI, told me and I was shocked, confused and felt like my World has crumbled. I was dying slowly due to the Announcement of my Medical Practitioner but he assured me that I could leave a normal life if I took my Medications (as there was no Medically known Cure to Herpes). I went from Churches to Churches but soon found that my Case needed urgent Attention as I was growing lean due to fear of dying anytime soon. In a bid to look for a lasting solution to my Predicament, I sought for solutions from the Voodoo World. I went online and searched for every Powerful Trad-medical Practitioner that I could Severe, cos I heard that the African Voodoo Priests had a cure to the Herpes Syndrome. It was after a little time searching the Web that I came across one Dr EHI (A Powerful African Voodoo Priest), Who offered to help me at a Monetary fee. I had to comply as this was my Final bus-stop to receiving a perfect healing. My last resolve was to take my life by myself, should this plan Fail. At last it worked out well. He gave me some steps to follow and I meticulously carried out all his Instructions. Last month, to be precise, I went back to the hospital to conduct another test and to my Amazement, the Results showed that " I am NEGATIVE". You can free yourself of this Herpes virus by consulting this great African Voodoo Priest via this e-mail:(drehicurehome123@gmail.com) or call and whats-app him on +2349030589283 He will help you and his Herbal Medication is Sure. He has the Cure on all this Disease/Virus as He told me and it listed. GENITAL WARTS/ PREGNANCY. EPILEPSY. GENPILENCIN, HIV/AIDS, MADNESS, WEAK ERECTION, WATERY SPERM, TUBERCULOUSES, PENIS ENLARGEMENT, PREGNANCY, DIABETICS, STROKE, EXPANDS OF PENIS BREAST H.P.V TYPE 1 TYPE 2 TYPE 3 AND TYPE 4. TYPE 5, HUMAN PAPAILOMA VIRUS, HERPES, SYPHILIS, BREAST CANCER, HEPATITIS A B and C, HIGH BLOOD PRESSURE, AND BODY BOILS, BODY ODDUR, AND BODY DISEASES , THANK TO YOU ALL FOR YOUR TIME OF READING MY TESTIMONY AND MOST ESPECIALLY YOU Dr EHI. I PRAY TO GOD TO NEVER LET ME AND MY FAMILY FORGET YOU IN OUR LIFE

    Why go the Expensive Treatment? when Dr. EHI is there to help you get rid of that
    Incurable Disease with his Herbs and Healing Oil. I contracted Genital Herpes 2 years ago, I saw this Awesome Testimonies you can view on google during my research for Herpes Cure from an article made by Alexander Millin and Lancelot Morgan, How they but got Cured from HIV/HERPES VIRUS Respectively. They also dropped the Contact Address of this Great Man: drehicurehome123@gmail.com Advising that if anyone need help to Cure any Incurable Disease or Viral Infection that he should Contact This Great Man. I contacted him, thank God I did. I'm still in shock that the Herbal Treatment he sent to me actually Works. It seems so simple, and in a lots of ways I guess its simple. But the important thing is that I have been 100% herpes free for full two years now and it's all thanks to you Dr. Ehi You'hv given me my life back, I'll forever remain Grateful,. you can email me for assistant on: drehicurehome123@gmail.com. Please Once you are cured let others know about it no need hiding it. Together We fight to become healthy.
    Dr. EHI Root/Herbs and Healing-Oil has been used to cured Home and Abroad
    REVELATION22.2
    GENESIS1:29

    ReplyDelete


  7. Am jeremiah, I am testifying about a great hebal man that cured my wife of hepatitis B and liver cirhosis. his name is Dr oniha. My wife was diagnose of hepatities two years ago, i almost spent all i had then, until i saw dr oniha recommendation online, and i call him, then he told me how to get the herb. You can call him on +2347089275769 or email him at dronihaspell@yahoo.com

    ReplyDelete
  8. Are you going through GENITAL HERPES? OR GENITAL WARTS? Dr. Iyabiye is the solution to your problem and I am a testimony to his great work. I suffered genital herpes and warts for a long time until 2 months ego when I read about his recommendation online on how he cured someone who went through the same problem. I contacted him and he sent me the medicine and after taking the medication for a month the pains where gone and I became healed /cured, I went to the hospital and I was tested negative which means I am free. You too can be free, reach him through: iyabiyehealinghome@gmail.com Call / Whatsapp: +2348072229413

    ReplyDelete
  9. The Medical doctors said Herpes virus does not have a medical cure because the virus is capable of hiding within the human cells, it remains protected from your immune system. Herpes isn’t a special virus – your immune system has the tools to fight it back. But because it is able to lay dormant in protected cells, your immune system is unable to remove it from your body,But with strong reactive herbal medication is capable of getting rid of the virus gradually and totally from your body without damaging any of your cells,natural herbs kills the virus totally not just reducing the outbreak. Get natural herbs cure from Dr Osalu, I'm a medical Doctor and i have tried this herb from Dr Osalu and it works very well, i will recommend everyone out there to try this herbal medicine from Dr Osalu, I will drop his contacts so that you can reach him all by yourself You can Email him via his email drosaluherbalhome@gmail.com for easy and fast communication you can also call or add him on whats-app with this number +2348078668950.

    ReplyDelete
  10. There are some natural remedies that can be used in the prevention and eliminate diabetes totally. However, the single most important aspect of a diabetes control plan is adopting a wholesome life style Inner Peace, Nutritious and Healthy Diet, and Regular Physical Exercise. A state of inner peace and self-contentment is essential to enjoying a good physical health and over all well-being. The inner peace and self contentment is a just a state of mind.People with diabetes diseases often use complementary and alternative medicine. I diagnosed diabetes in 2000. Was at work feeling unusually tired and sleepy. I borrowed a glucometer from a co-worker and tested at 760. Went immediately to my doctor and he gave me prescibtion like: Insulin ,Sulfonylureas,Thiazolidinediones but Icould not get the cure rather to reduce the pain abd brink back the pain again. i found a woman testimony name Comfort online how Dr Akhigbe cure her HIV  and I aslo contacted the doctor and after I took his medication as instructed, I am now completely free from diabtes by doctor Akhigbe herbal medicine.So diabetes patients reading this testimony to contact his email     drrealakhigbe@gmail.com   or his Number   +2348142454860   He also use his herbal herbs to diseases like:SPIDER BITE, SCHIZOPHRENIA, LUPUS,EXTERNAL INFECTION, COMMON COLD, JOINT PAIN, EPILEPSY,STROKE,TUBERCULOSIS ,STOMACH DISEASE. ECZEMA, PROGERIA, EATING DISORDER, LOWER RESPIRATORY INFECTION,  DIABETICS,HERPES,HIV/AIDS, ;ALS,  CANCER , MENINGITIS,HEPATITIS A AND B,ASTHMA, HEART DISEASE, CHRONIC DISEASE. NAUSEA VOMITING OR DIARRHEA,KIDNEY DISEASE.Dr Akhigbe is a good man and he heal any body that come to him. here is email    drrealakhigbe@gmail.com    and his Number +234814245486

    ReplyDelete
  11. i already gave up on ever getting cured of HSV2 because i have try many treatment none of them work out for me i have go to different hospital they always tell me same thing there is no cure for herpes when i came across a post about Dr Goodwin, in the net from a lady called Angela i contacted him and he reassured me with him herbal medicine which i took according to the way he instructed, that how i was cured. I doubted at first because i have been to a whole lot of reputable doctors, tried a lot of medicines but none was able to cure me. so i decided to listen to him and he commenced treatment, and under two weeks i was totally fee from #Herpes. i want to say a very big thank you to DR godwin for what he has done in my life. feel free to leave him a message on email drgodwinsolutionhome@gmail.com and also WhatsApp him +2348054615060..
    He can still be able to help you with this herbs medicine:
    1...ALS CURE/DIABETES CURE/EPILESY/HPV

    ReplyDelete
  12. I'm 57 years old and female. I was diagnosed a couple of years ago with COPD and I was beyond scared! My lung function test indicated 49% capacity. After having had flu a year ago, the shortness of breath, coughing and chest pains continued even after being treated with antibiotics. I've been smoking two packs a day for 36 years. Being born without a sternum caused my ribs to be curled in just one inch away from my spine, resulting to underdeveloped lungs. At age 34 I had surgery and it was fixed. Unfortunately my smoking just caused more damage to my already under developed lungs. The problem was having is that I enjoy smoking and don't want to give up! Have tried twice before and nearly went crazy and don't want to go through that again. I saw the fear in my husband and children's eyes when I told them about my condition then they start to find solution on their own to help my condition.I am an 57 now who was diagnose COPD emphysema which I know was from my years of smoking. I started smoking in school when smoking was socially acceptable. I remember when smoking was permitted in hospitals. It was not known then how dangerous cigarettes were for us, and it seemed everybody smoked but i was able to get rid of my COPD lung condition through the help of  Dr Akhigbe   total cure herbal medicine. my husband saw his testimony on the internet he used his powerful medicine to cured different diseases. we contacted his email   [drrealakhigbe@gmail.com}   He has the right herbal formula to help you get rid and repair any lung conditions and others diseases, will cure you totally and permanently with his natural organic herbs,We received the medicine through courier delivery service. I wish anybody who starts smoking at a young age would realize what will eventually happen to their bodies if they continue that vile habit throughout their life.
    Dr Akhighe also cured diseases like, HERPES, DIABETES, HIV/AIDS, COPD, CANCER,ASTHMA,STROKE,LUPUS,JOINT PAIN,CHRONIC DISEASES,PARKINSON DISEASES,TUBERCULOSIS,HIGH BLOOD PRESSURE,BREAST INFECTION,WOMEN SEXUAL PROBLEM, GINGIVITIS, ERYSIPELAS,STAPHYLOCOCCUS,HEPATITIS A/B, QUICK EJACULATION, IMMUNOTHERAPY, GONORRHEA,SYPHILIS,WAST/BACK PAIN,PELVIC INFLAMMATORY, DICK ENLARGEMENT,HEART DISEASES,TERMINAL ILLNESS,SHIFT IN FOCUS,ATAXIA,COMMON COLD,CROHN'S DISEASES,ALCOHOL SPECTRUM DISORDER,GRAVES DISEASE,HEARING LOSS, INTERSTITIA CYSTITIS,LEUKEMIA,MULTIPLE SCLEROSIS,OBESITY,RABIES,SCOLIOSIS,INFLUENZA, POLIO,JACOB,ETC. If you are out there looking for your cure please  contact  dr Akhigbe  by his email    drrealakhigbe@gmail.com     or contact his whatsapp number   +2349010754824
    God bless you Dr Akhigbe for your good hand work on my life.

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