Tuesday, February 21, 2012

Hepatic Insulin by Elsner at

The plan for this research was to genetically engineer a virus to create insulin, and then inject that virus into the liver of diabetic animals.  The result was normal BG for the one year duration of the trial.

Title: Reversal of Diabetes Through Gene Therapy of Diabetic Rats by Hepatic Insulin Expression via Lentiviral Transduction

Quotes from Abstract:
  • Within 5–7 days after the virus injection of 7 × 109INS-lentiviral particles the blood glucose concentrations were normalized in the treated animals. 
  • This glucose lowering effect remained stable for the 1 year observation period. 
  • Human C-peptide as a marker for hepatic release of human insulin was in the range of 50–100 pmol/ml serum.
  • This study shows that the diabetic state can be efficiently reversed by insulin release from non-endocrine cells through a somatic gene therapy approach.
Two different types of animals were used:

  • autoimmune-diabetic IDDM rats (LEW.1AR1/Ztm-iddm) 
  • streptozotocin-diabetic rats

Abstract: http://www.ncbi.nlm.nih.gov/pubmed/22354377

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