Tuesday, February 21, 2012

Hepatic Insulin by Elsner at

The plan for this research was to genetically engineer a virus to create insulin, and then inject that virus into the liver of diabetic animals.  The result was normal BG for the one year duration of the trial.

Title: Reversal of Diabetes Through Gene Therapy of Diabetic Rats by Hepatic Insulin Expression via Lentiviral Transduction

Quotes from Abstract:
  • Within 5–7 days after the virus injection of 7 × 109INS-lentiviral particles the blood glucose concentrations were normalized in the treated animals. 
  • This glucose lowering effect remained stable for the 1 year observation period. 
  • Human C-peptide as a marker for hepatic release of human insulin was in the range of 50–100 pmol/ml serum.
  • This study shows that the diabetic state can be efficiently reversed by insulin release from non-endocrine cells through a somatic gene therapy approach.
Two different types of animals were used:

  • autoimmune-diabetic IDDM rats (LEW.1AR1/Ztm-iddm) 
  • streptozotocin-diabetic rats

Abstract: http://www.ncbi.nlm.nih.gov/pubmed/22354377

Tuesday, February 7, 2012

Brown Adipose Tissue Transplant by Gunawardana at Vanderbilt University

Title: Reversal of Type 1 Diabetes in Mice by Brown Adipose Tissue Transplant

Quote from the abstract:
Here, we show that subcutaneous transplants of embryonic brown adipose tissue (BAT) can correct T1D in streptozotocin-treated mice (both immune competent and immune deficient) ... BAT transplants result in euglycemia, normalized glucose tolerance, reduced tissue inflammation, and reversal of clinical diabetes markers such as polyuria, polydipsia, and polyphagia.