The plan for this research was to genetically engineer a virus to create insulin, and then inject that virus into the liver of diabetic animals. The result was normal BG for the one year duration of the trial.
Title: Reversal of Diabetes Through Gene Therapy of Diabetic Rats by Hepatic Insulin Expression via Lentiviral Transduction
Quotes from Abstract:
Two different types of animals were used:
- Within 5–7 days after the virus injection of 7 × 109INS-lentiviral particles the blood glucose concentrations were normalized in the treated animals.
- This glucose lowering effect remained stable for the 1 year observation period.
- Human C-peptide as a marker for hepatic release of human insulin was in the range of 50–100 pmol/ml serum.
- This study shows that the diabetic state can be efficiently reversed by insulin release from non-endocrine cells through a somatic gene therapy approach.
- autoimmune-diabetic IDDM rats (LEW.1AR1/Ztm-iddm)
- streptozotocin-diabetic rats