Saturday, March 2, 2013

anti-CD20 and anti-CD3 by Hu and Wen at Yale


Combination treatment with anti-CD20 and oral anti-CD3 prevents and reverses autoimmune diabetes

Abstract: Type 1 diabetes (T1D) is a T cell-mediated autoimmune disease, although B cells also play an important role in T1D development. Both T cell- and B cell-directed immunotherapies have shown efficacy in the prevention and reversal of T1D. However, whether the combined strategy of targeting both T and B cells could further improve the therapeutic efficacy remains to be explored. Herein we show that the combined treatment with intravenous anti-human CD20 (hCD20) and oral anti-CD3 significantly delayed diabetes development in pre-diabetic hCD20 transgenic NOD mice. More importantly, the combined treatment reversed diabetes in more than 60% of mice newly diagnosed with diabetes. Further mechanistic studies demonstrated that the addition of oral anti-CD3 to the B cell depletion therapy synergistically enhanced the suppressive function of Treg. Interestingly, the oral anti-CD3 treatment induced a fraction of IL-10-producing CD4 T cells in the small intestine through IL-10 and IL-27-producing dendritic cells. Thus, our findings demonstrated that combining anti-CD20 and oral anti-CD3 is superior to anti-CD20 monotherapy for restoring normoglycemia in diabetic NOD mice, providing important preclinical evidence for the optimization of B cell-directed therapy for type 1 diabetes.

9 comments:

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  4. mercy is my name; i had suffered Diabetes for several years ago. My sugar level was as high as anything you can think about. i looked for how to get rid of this diabetes for a long period of time, but could not found any solution. Though i have always believed on herbal medicine, but was never chanced to look for any because of my work. But not too long i saw some good news on the internet on how a herbal doctor cured so many persons of various diseases, and viruses. So i also decided to contact the doctor those people talked about he is by the name Doctor Aba. I emailed him, and i explained my problem to him, he said he has the cure for diabetes and i decided to give him a try because there is no harm in trying. So i started the process, and he prepared some herbs and sent it to me here in my country, so i began to use it, and behold it worked for me like a magic. I am happy to say today i am diabetes free, and my sugar level is back to normalcy. Please if you also have same, and or similar problem please rush to contact Doctor Aba before it becomes too late for you. His email is dr.abaherbalhome@gmail.com you can whats App his number on +2348107155060

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    Why go the Expensive Treatment? when Dr. EHI is there to help you get rid of that
    Incurable Disease with his Herbs and Healing Oil. I contracted Genital Herpes 2 years ago, I saw this Awesome Testimonies you can view on google during my research for Herpes Cure from an article made by Alexander Millin and Lancelot Morgan, How they but got Cured from HIV/HERPES VIRUS Respectively. They also dropped the Contact Address of this Great Man: drehicurehome123@gmail.com Advising that if anyone need help to Cure any Incurable Disease or Viral Infection that he should Contact This Great Man. I contacted him, thank God I did. I'm still in shock that the Herbal Treatment he sent to me actually Works. It seems so simple, and in a lots of ways I guess its simple. But the important thing is that I have been 100% herpes free for full two years now and it's all thanks to you Dr. Ehi You'hv given me my life back, I'll forever remain Grateful,. you can email me for assistant on: drehicurehome123@gmail.com. Please Once you are cured let others know about it no need hiding it. Together We fight to become healthy.
    Dr. EHI Root/Herbs and Healing-Oil has been used to cured Home and Abroad
    REVELATION22.2
    GENESIS1:29

    ReplyDelete
  8. I'm Dorothy Saltzman, From Texas, USA. but curently Reside in Germany. It's no longer Rumors that the Acquired Immune Deficiency Syndrome ALS And Herpes Virus is increasing Day by Day. The fear is that many people living with the sickness are scared of saying it because of the stigma that comes along with it. I 'm bold enough among many others to state that there is now a Potent Cure to this sickness but many are unaware of it. I discovered that I was infected with ALS virus 3months Ago, After a Herbal Medical Check-Up. My Doctor EHI, told me and I was shocked, confused and felt like my World has crumbled. I was dying slowly due to the Announcement of my Medical Practitioner but he assured me that I could leave a normal life if I took my Medications (as there was no Medically known Cure to Herpes). I went from Churches to Churches but soon found that my Case needed urgent Attention as I was growing lean due to fear of dying anytime soon. In a bid to look for a lasting solution to my Predicament, I sought for solutions from the Voodoo World. I went online and searched for every Powerful Trad-medical Practitioner that I could Severe, cos I heard that the African Voodoo Priests had a cure to the Herpes Syndrome. It was after a little time searching the Web that I came across one Dr EHI (A Powerful African Voodoo Priest), Who offered to help me at a Monetary fee. I had to comply as this was my Final bus-stop to receiving a perfect healing. My last resolve was to take my life by myself, should this plan Fail. At last it worked out well. He gave me some steps to follow and I meticulously carried out all his Instructions. Last month, to be precise, I went back to the hospital to conduct another test and to my Amazement, the Results showed that " I am NEGATIVE". You can free yourself of this Herpes virus by consulting this great African Voodoo Priest via this e-mail:(drehicurehome123@gmail.com) or call and whats-app him on +2349030589283 He will help you and his Herbal Medication is Sure. He has the Cure on all this Disease/Virus as He told me and it listed. GENITAL WARTS/ PREGNANCY. EPILEPSY. GENPILENCIN, HIV/AIDS, MADNESS, WEAK ERECTION, WATERY SPERM, TUBERCULOUSES, PENIS ENLARGEMENT, PREGNANCY, DIABETICS, STROKE, EXPANDS OF PENIS BREAST H.P.V TYPE 1 TYPE 2 TYPE 3 AND TYPE 4. TYPE 5, HUMAN PAPAILOMA VIRUS, HERPES, SYPHILIS, BREAST CANCER, HEPATITIS A B and C, HIGH BLOOD PRESSURE, AND BODY BOILS, BODY ODDUR, AND BODY DISEASES , THANK TO YOU ALL FOR YOUR TIME OF READING MY TESTIMONY AND MOST ESPECIALLY YOU Dr EHI. I PRAY TO GOD TO NEVER LET ME AND MY FAMILY FORGET YOU IN OUR LIFE

    Why go the Expensive Treatment? when Dr. EHI is there to help you get rid of that
    Incurable Disease with his Herbs and Healing Oil. I contracted Genital Herpes 2 years ago, I saw this Awesome Testimonies you can view on google during my research for Herpes Cure from an article made by Alexander Millin and Lancelot Morgan, How they but got Cured from HIV/HERPES VIRUS Respectively. They also dropped the Contact Address of this Great Man: drehicurehome123@gmail.com Advising that if anyone need help to Cure any Incurable Disease or Viral Infection that he should Contact This Great Man. I contacted him, thank God I did. I'm still in shock that the Herbal Treatment he sent to me actually Works. It seems so simple, and in a lots of ways I guess its simple. But the important thing is that I have been 100% herpes free for full two years now and it's all thanks to you Dr. Ehi You'hv given me my life back, I'll forever remain Grateful,. you can email me for assistant on: drehicurehome123@gmail.com. Please Once you are cured let others know about it no need hiding it. Together We fight to become healthy.
    Dr. EHI Root/Herbs and Healing-Oil has been used to cured Home and Abroad
    REVELATION22.2
    GENESIS1:29

    ReplyDelete